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Image Search Results
Journal: Molecular Medicine Reports
Article Title: Identification of a novel anti-heat shock cognate 71 kDa protein antibody in patients with Kawasaki disease
doi: 10.3892/mmr.2020.10973
Figure Lengend Snippet: Indirect immunofluorescence assay with HeLa cell chips. Serum samples containing the probe antibodies were incubated with the cell chips, which were visualized using an Olympus FV1000 confocal laser-scanning microscope at ×40 magnification. (A) KD serum exhibited significantly higher immunoreactivity with proteins from HeLa cells compared with (B) FC and (C) HC serum. (D) Positive control (serum samples replaced with anti-ACTB antibody) and (E) blank control (serum samples replaced with PBS) groups. (F) Semi-quantitative analysis of the fluorescence intensity of KD, FC, and HC. Positive recognition of proteins in the HeLa cells by antibodies in the serum appear green, and the nucleus is stained blue. ACTB, β-actin; FC, febrile control; HC, healthy control; KD, Kawasaki disease.
Article Snippet: Images of cell chips were captured using an Olympus
Techniques: Immunofluorescence, Incubation, Laser-Scanning Microscopy, Positive Control, Control, Fluorescence, Staining